U etsa bonnete ba hore maemo a nyopa ha u sebelisa mekotla ho microbiology?

Bohlokoa ba Sampling e Sterile ho Microbiology

Ho microbiology, ho boloka maemo a nyopa nakong ea pokello ea sampole le lipalangoang ho bohlokoa ho netefatsa bonnete le ts'epahalo ea liphetho tsa liteko. Mekotla e se nang likokoana-hloko e etselitsoeng ts'ebeliso ea likokoana-hloko e na le karolo ea bohlokoa ho finyella sena. Mekotla ena e thibela tšilafalo ho tsoa mehloling ea kantle ea likokoana-hloko, e boloka botšepehi ba lisampole tse kang lisele tsa liphoofolo, lijo, metsi le lisampole tse fapaneng tsa tikoloho. Ho sebelisa mekotla e hloekisitsoeng ho fokotsa kotsi ea tšoaetso, kahoo ho boloka bonnete ba lisampole tse bokelletsoeng le lintlha tse nkiloeng ho tsona.

Ho Khetha Mekotla e Loketseng e Opang bakeng sa Sampling

Ho Nahana ka Lintho Tse Bonahalang

Khetho ea mekotla e hloekisitsoeng e lokela ho ipapisa le bokhoni ba thepa ea ho boloka bohloeki le ho thibela tšoaetso. Lisebelisoa tse tloaelehileng li kenyeletsa polyethylene e phahameng - Ha u khetha mekotla, nahana ka mofuta oa sampole le maemo a tikoloho ho netefatsa ts'ebetso e nepahetseng.

Moralo le Likarolo

Mekotla e sterile hangata e na le thepa e ntle ea ho tiisa ho boloka sampole ea botšepehi. Meralo ea lifeme tse tsoang ho bahlahisi ba tsebahalang ba Chaena e totobatsa likhetho tse bobebe le tse bonolo-ho-ho li jara, li etsa hore li tšoanelehe bakeng sa lisampole. Mekotla e lokela ho ba le likarolo tse kang tamper-litiiso tse hlakileng le libaka tse hlakileng tsa ho ngola ho ntlafatsa ts'ebeliso.

Tokisetso e Nepahetseng ea Pokello ea Mehlala

Ho Khetha Libaka tsa Sampling

Ho bohlokoa ho khetholla libaka tse nepahetseng tsa lisampole ho qoba tšilafalo ea tikoloho. Sebaka se lokela ho khethoa ho latela mofuta oa sampole le mehloli e ka bang teng ea tšilafalo.

Ho bokella lisebelisoa tse hlokahalang

Pele o nka sampole, bokella lisebelisoa tsohle tsa bohlokoa, ho kenyeletsoa liatlana, li-sanitizer tsa letsoho, le lihlapa tse bolaeang likokoana-hloko. Netefatsa hore lisebelisoa tsohle tse sebelisoang li hloekisitsoe ho thibela ho hlahisa litšila nakong ea lisampole.

Katleho On-Site Sampling Techniques

On-site sampole e hloka ho nepahala ho netefatsa hore disampole li boloka boemo ba tsona ba pele. Hloekisa sebaka sa ho etsa lisampole ka lihlakola tse bolaeang likokoana-hloko ho thibela tšilafalo ea tikoloho. Nakong ea ts'ebetso ena, mekotla e sa hloekang e netefatsa hore ha ho likokoana-hloko kapa likaroloana tse kena-kenanang le sampuli, ho boloka bohloeki ba eona ho tloha ho bokella ho ea ho tlhahlobo.

E Sireletsehile ebile e Silafetse-Ts'ebetso ea Mehlala ea Mahala

Sample Pokello Protocol

Sebelisa lisebelisoa tsa sampole tse hloekisitsoeng ho bokella sampole, ho netefatsa hore ha ho kopane ka letsoho ka kotloloho. Sena se fokotsa tšoaetso ea baktheria. Sampole e lokela ho kenngoa ka ho toba ka mokotleng o sa hloekang, ho netefatsa hore ho na le moea o fokolang ka ho ntša moea o feteletseng pele o koala.

Ho Tiisa le ho Ngola

Hang ha sampole e sirelelitsoe ka mokotleng o hloekisitsoeng, e ngole hang-hang ka lintlha tsohle tse hlokahalang. Netefatsa hore mokotla o koetsoe ka botlalo ho thibela ho lutla kapa ho pepeseha, ho ka behang sterility ea sampole kotsing.

Ho boloka lisampole ka mekotleng e nyopa

Mekotla e sa hloekang e etselitsoe ho boloka boemo ba lisampole nakong ea lipalangoang le polokelo. Ho tiisoa ka nepo ho thibela ho lutla le ho pepesehela lintlha tse kantle, ho netefatsa botšepehi ba sampole e lula e tiile ho tloha sebakeng sa pokello ho ea tlhahlobong ea laboratori.

Lipalangoang le polokelo ea lisampole tse nyopa

Ho nkeha le ho sebetsa ka katleho

Moralo oa mekotla e hloekisitsoeng e thusa ho tsamaea ha bonolo, e leng ntlha ea bohlokoa ho bafuputsi ba masimong. Mekotla ena, eo hangata e rekoang ho tsoa lifekthering tsa China, e entsoe hore e be bobebe hape e be matla, e nolofalletsang lipalangoang tse sebetsang hantle le tse bolokehileng ho ea lilaboratoring.

Maemo a polokelo

Boloka disampole maemong a bolokang botshepehi ba tsona, hangata ho mocheso-tikoloho e laoloang. Maemo a polokelo a lokela ho lumellana le mofuta oa mohlala ho thibela ho senyeha kapa ho silafala.

Mehato ea Tšireletseho ea ho Tšoara Mekotla e Hlapang

Litsebi tsa baeloji li tlameha ho latela melaoana e tiileng ea tšireletso ha li tšoara mekotla e se nang likokoana-hloko, haholo-holo ha lisampole li na le likokoana-hloko tse kotsi. Ts'ebeliso ea lisebelisoa tsa ts'ireletso ea motho (PPE) e theha tšitiso, e fokotsang kotsi ea tšoaetso le ho netefatsa polokeho ea basebelisi.

Netefatso ea Boleng ho Tlhahiso ea Mokotla o Seretse

Tlhahiso ea lifeme tsa mekotla e hloekisitsoeng e kenyelletsa mehato e thata ea taolo ea boleng ho netefatsa ho beleha. Hangata bahlahisi ba Machaena ba sebelisa liphekolo tse phahameng - mocheso le holimo - khatello ea maikutlo ho netefatsa hore mekotla e silafetse Mekhoa e tsitsitseng ea netefatso ea boleng e bohlokoa ho netefatsa hore mekotla e sa tsoakoang e fihlela litekanyetso tse hlokahalang tsa machaba tsa likokoana-hloko.

Ho bapisa Aseptic vs. Sterile Techniques

Ho utloisisa phapang lipakeng tsa mekhoa ea aseptic le e sterile ho bohlokoa bakeng sa ho boloka botšepehi ba sampole. Le hoja mekhoa e hloekisitsoeng e felisa mefuta eohle ea bophelo ba likokoana-hloko, mekhoa ea aseptic e kenyelletsa mekhoa e thibelang tšoaetso. Mekhoa ena ka bobeli e bohlokoa sebakeng sa laboratori ho netefatsa ho ts'epahala ha sephetho le polokeho ea lits'ebetso tsa ho sebetsana le likokoana-hloko.

Kedun Fana ka Litharollo

Kedun e fana ka mefuta e mengata ea mekotla e hloekisitsoeng ea boleng bo holimo e etselitsoeng ho kopana le maemo a thata a likokoana-hloko. Lihlahisoa tsa rona li entsoe ka tlas'a taolo e matla ea boleng seterekeng sa rona - sa - meaho ea bonono ea China. Re fana ka lits'ebeletso tse rekisoang ka bongata, ho netefatsa hore bareki ba rona ba fumana litharollo tse tšepahalang le tse sebetsang hantle bakeng sa litlhoko tsa bona tsa sampole ea likokoana-hloko. Boitlamo ba rona ba bokhabane bo tiisa ho hloeka le ho tšoarella ha mekotla ea rona, ho kenya letsoho katlehong ea lipatlisiso le liteko tsa hau tsa likokoana-hloko.

Patlisiso e chesang ea basebelisi:mekotla e nyopa bakeng sa microbiology