Chikwama Chowonjezera Zitsanzo: Siponji ya Kedun - Ndodo yokhala ndi 10mL Neutralizing Buffer

Dziwani zaluso lopanda msoko la zitsanzo zamadzimadzi ndi Siponji yosinthira ya Kedun - Ndodo yokhala ndi 10 mL Neutralizing Buffer, yopangidwira kukhathamiritsa ndi kufewetsa njira yanu yotsatsira. Kuzindikiridwa ngati chizindikiro pazasampuli zamadzimadzi, chikwama chathu chosonkhanitsira zitsanzo chapangidwa mwatsatanetsatane kuti zitsimikizire kubweza ndi kusungirako zitsanzo zoyenera. Ntchito yayikulu ya thumba lathu lotolera zitsanzo ndikuwongolera kusonkhanitsa zitsanzo zamadzimadzi, zomwe zikuwonetsa kuti ndizofunikira kwambiri m'ma laboratories, mabungwe ofufuza, ndi mabungwe osiyanasiyana ofufuza. Mwapadera pamapangidwe ake, chikwamacho chimakhala ndi Siponji - Ndodo, chopereka chida chodabwitsa chomwe chimathandizira kusonkhanitsa bwino zitsanzo. Kuphatikizidwa ndi 10 ml Neutralizing Buffer, Siponji-Ndodo imawonetsetsa kuti chitsanzocho chikhalabe chosadetsedwa komanso chosakhudzidwa panthawi yosonkhanitsa, motero zimatsimikizira chiyero ndi kukhulupirika kwa chitsanzo chanu. Chikwamacho chimapangidwa kuti chikhale cholimba komanso chokhazikika, kuwonetsetsa kuti zitsanzo zanu zimakhalabe bwino komanso zosasunthika panthawi yoyendetsa ndi kusunga.

Ntchito ya Kedun Filter Sampling Bag ndikuthandizira kusonkhanitsa zitsanzo zamadzimadzi. Tinthu tating'onoting'ono timasiyanitsidwa ndi zitsanzo zamadzimadzi ndi nembanemba ya fyuluta pogwiritsa ntchito thumba lachitsanzo la fyuluta kuyeretsa zitsanzo. Zopanda particles zolimba zimathetsa chiopsezo cha pipette kutsekeka kwa zitsanzo zoyera ndi kugawa mbale.

ade kuchokera ku polyethylene yapamwamba kwambiri, matumba a zitsanzo za Kedun ndi puncture-umboni woletsa kutayikira kwachitsanzo panthawi ya homogenization. Amapereka malo otetezeka, aseptic komanso osinthika a zitsanzo kuti atsimikizire kuti palibe chikoka pa kusanthula kwachitsanzo. Chidutswa chimodzi cha sefa chimakhazikika mbali ziwiri ndi pansi pa thumba la zitsanzo. Kukula kwa bowo pa dzenje lililonse ndi pafupifupi ma microns 350 ndipo kuchuluka kwa mabowo ndi 2,240 pa inchi imodzi.

Ndibwino pindani matepi katatu mutatolera zitsanzo m'matumba kuti asindikize bwino. Pali malo oyera omwe amasindikizidwa pa thumba kuti ogwiritsa ntchito alembe zambiri. Malo olembera oyera pathumba lachitsanzo chosabala ndi osankha. Ponyamula zitsanzo zambiri, waya wophwanthira adapangidwa kuti athandizire kukhazikika kwamatumba akuluakulu, mwachitsanzo matumba a 55 oz.

Matumba onse a Kedun sampling samples ndi osawilitsidwa ndi DNase-zaulere, RNase-zaulere, Pyrogen yaulere.

The Sponge-Stick with the 10mL Neutralizing Buffer imayikadi chikwama chathu chosonkhanitsira zitsanzo padera, ndikuwonjezera chitetezo ndi kudalirika pamasankho anu. Kedun adadzipereka kupereka mayankho abwino kwambiri komanso otsogola kwa makasitomala athu, ndipo chikwama chathu chotolera zitsanzo chili ngati umboni wa kudzipereka kumeneku. Munthawi yomwe kulondola komanso kuchita bwino ndizofunikira kwambiri, thumba lachitsanzo la Kedun limapereka yankho lomwe limaphatikiza izi mu chinthu chimodzi, chosavuta-kugwiritsa ntchito. Ndi mawu opitilira 800 odalirika komanso odalirika, tikukupemphani kuti mudzawone momwe siponji yathu imagwirira ntchito - Ndodo yokhala ndi 10mL Neutralizing Buffer. Sinthani momwe mumasonkhanitsira zitsanzo zanu ndi Kedun - chifukwa zambiri zonse ndi zofunika.