Ibhegi eyomeleziweyo yokuqokelelwa kweSampuli: Isiponji sikaKedun- Intonga ene-10mL eNeutralizing Buffer

Fumanisa ubugcisa obungenamthungo besampulu yolwelo kunye neSiponji soguqulo sikaKedun-Intonga ene-10 mL Neutralizing Buffer, eyilelwe ukukhulisa kunye nokwenza lula inkqubo yakho yesampulu. Iqondwa njengophawu kwindawo yesampulu yolwelo, ingxowa yethu yokuqokelela isampulu yenziwe ngobuchwephesha obucokisekileyo ukuze kuqinisekiswe ukufunyanwa kwesampulu kunye nokugcinwa kwayo. Umsebenzi ophambili wengxowa yokuqokelela isampulu yelizwe lethu kukulungelelanisa ukuqokelelwa kweesampulu zolwelo, ezibonisa ukuba yi-asethi eyimfuneko kwiilebhu, kumaziko ophando kunye namaziko ophando. Eyodwa kuyilo lwayo, ibhegi iza ixhotyiswe ngeSponge- Intonga, ibonelela ngesixhobo esimangalisayo esinceda ekuqokeleleni iisampulu ngokufanelekileyo. Idityaniswe ne-10 ml ye-Neutralizing Buffer, i-Sponge-Intonga iqinisekisa ukuba isampulu ihlala ingangcolisekanga kwaye ingathathi hlangothi ngexesha lenkqubo yokuqokelela, ngaloo ndlela iqinisekisa ukucoceka kunye nokuthembeka kwesampulu yakho.Nokuba uqhuba uphando lwezemfundo, uqhuba uvavanyo lwamashishini, okanye uqhuba iimviwo ze-forensic, ingxowa yokuqokelela iisampulu ye-Kedun ifanelekile ngokufanelekileyo ukuhlangabezana neemfuno zakho. Ibhegi yenzelwe ukuba yomelele kwaye yomelele, iqinisekisa ukuba iisampulu zakho zihlala zilungile kwaye zingenakuchaphazeleka ngexesha lothutho kunye nokugcinwa.

Umsebenzi we-Kedun Filter Sampling Bag kukwenza lula ukuqokelelwa kweesampulu zolwelo. Amasuntswana aqinileyo ahlulwe kwiisampuli zolwelo ngenwebu yokucoca xa usebenzisa ingxowa yesampuli yokucoca ukucoca iisampulu. I-Free of particles eqinile isusa umngcipheko wokuvalwa kwe-pipette kwiisampuli ezicocekileyo kunye nokuhanjiswa kweplate.

I-ade evela kwi-polyethylene ekumgangatho ophezulu, iingxowa zesampulu ze-Kedun zokucoca i-puncture-ubungqina bokuthintela ukuvuza kwesampulu ngexesha le-homogenization. Inika indawo ekhuselekileyo, i-aseptic kunye neguquguqukayo yesampulu ukuqinisekisa ukuba akukho mpembelelo kuhlalutyo lwesampulu. Iqhekeza elinye le-membrane yokucoca igxininiswe kumacala amabini kunye nezantsi kwengxowa yesampuli. Ubungakanani bomngxuma womngxuma ngamnye bujikeleze i-microns ezingama-350 kwaye ubungakanani bemingxuma yi-2,240 nge-intshi nganye yesikwere.

Kucetyiswa ukuba ugobe amateyipu kathathu emva kokuqokelela iisampuli kwiingxowa zokutywinwa okungcono. Kukho indawo emhlophe eprintwe ebhegini ukuze abasebenzisi bamakishele ulwazi. Indawo yokumakisha emhlophe kwingxowa yesampulu engcolileyo ayikhethi. Ukubamba isixa esikhulu seesampulu, ucingo olusicaba luyilelwe ukunceda ukuzinzisa iingxowa ezinomthamo omkhulu, umzekelo iingxowa ezingama-55 oz.

Zonke iisampulu zeesampulu zeengxowa ze-Kedun ziyavalwa kwaye DNase-mahala, RNase-mahala, iPyrogen isimahla.

I-Sponge-Intonga ene-10mL Neutralizing Buffer ibeka ngenene ibhegi yethu yokuqokelela isampulu, yongeza umaleko owongezelelweyo wokhuseleko kunye nokuthembeka kwinkqubo yakho yesampulu. I-Kedun izimisele ukuhambisa umgangatho ophezulu kunye nezisombululo ezintsha kubathengi bethu, kwaye ibhegi yethu yokuqokelela isampulu imi njengobungqina bokuzibophelela. Ngexesha apho ukuchaneka kunye nokusebenza kakuhle kubaluleke kakhulu, ingxowa ye-sampulu ye-Kedun yokuqokelela ibonelela ngesisombululo esidibanisa ezi mpawu kwimveliso enye, kulula-ukusebenzisa. Ngamagama angaphezulu kwama-800 okuthembakala kunye nokuthembeka, siyakumema ukuba ube namava okusebenza okugqwesileyo kweSponji sethu - Intonga ene-10mL yeNeutralizing Buffer. Guqula indlela oqokelela ngayo iisampulu zakho ngeKedun - kuba zonke iinkcukacha zibalulekile.