Isikhwama Esithuthukisiwe Sokuqoqwa Kwesampula: Isiponji sika-Kedun-Induku ene-10mL Neutralizing Buffer

Zitholele ubuciko obungenamthungo besampula obuwuketshezi ngesiponji sika-Kedun esiguqukayo-Induku ene-10 mL Neutralizing Buffer, eklanyelwe ukuthuthukisa nokwenza lula inqubo yakho yokuthatha amasampula. Njengoba siqashelwa njengesilinganiso emkhakheni wamasampula oketshezi, isikhwama sethu sokuqoqa isampula siklanywe ngokunemba okucophelela ukuze kuqinisekiswe ukubuyiswa kwesampula okuphelele nokugcinwa.Umsebenzi oyinhloko wesikhwama sethu sokuqoqa isampula yesimo-se-ubuciko ukwenza kube lula ukuqoqwa kwamasampula oketshezi, okufakazela ukuthi kuyimpahla ebaluleke kakhulu kumalabhorethri, izikhungo zocwaningo, izikhungo zocwaningo, izikhungo zocwaningo. Ihlukile ekwakhiweni kwaso, isikhwama sifika sifakwe I-Sponge-Stick, esihlinzeka ngethuluzi elimangalisayo elisiza ekuqoqweni okuphumelelayo kwamasampuli. Ihlanganiswe ne-10 mL Neutralizing Buffer, i-Sponge-Stick iqinisekisa ukuthi isampula lihlala lingangcolisiwe futhi lingathathi hlangothi phakathi nenqubo yokuqoqa, ngaleyo ndlela iqinisekisa ubumsulwa nobuqotho besampula yakho. Kungakhathaliseki ukuthi wenza ucwaningo lwezemfundo, wenza ukuhlola kwemboni, noma wenza izivivinyo ze-forensic, isikhwama sesampula se-Kedun sifaneleka ngokufanelekile ukuze sihlangabezane nezidingo zakho. Isikhwama siklanyelwe ukuba siqine futhi siqine, siqinisekisa ukuthi amasampula akho ahlala eqinile futhi angenabungozi ngesikhathi sokuthutha nokugcinwa.

Umsebenzi we-Kedun Filter Sampling Bag ukwenza lula ukuqoqwa kwamasampula oketshezi. Izinhlayiya eziqinile zihlukaniswa kumasampula oketshezi ngolwelwesi lwesihlungi lapho kusetshenziswa isikhwama sesampula sokuhlunga ukuhlanza amasampula. Ukukhululeka kwezinhlayiya eziqinile kuqeda ingozi yokuvinjelwa kwe-pipette kumasampuli ahlanzekile nokusabalalisa kwamapuleti.

I-ade evela ku-polyethylene yekhwalithi ephezulu, izikhwama zokuhlunga ze-Kedun ziyi-puncture-ubufakazi bokuvimbela ukuvuza kwesampula ngesikhathi se-homogenization. Ihlinzeka ngesikhala esiphephile, se-aseptic nesiguquguqukayo sesampula ukuze kuqinisekiswe ukuthi akukho thonya ekuhlaziyweni kwesampula. Ucezu olulodwa lwe-membrane yesihlungi lugxilwe ezinhlangothini ezimbili nangaphansi kwesikhwama samasampula. Usayizi wembobo wembobo ngayinye ungama-microns angama-350 futhi inani lemigodi lingama-2,240 iyintshi yesikwele.

Kunconywa ukuthi ugoqe amateyipu izikhathi ezintathu ngemva kokuqoqa amasampula ezikhwameni ukuze avalwe kangcono. Kukhona indawo emhlophe ephrintwe esikhwameni ukuze abasebenzisi bamake ulwazi. Indawo yokumaka emhlophe esikhwameni samasampula oyinyumba iyakhethwa. Ukuze ubambe amasampula amaningi, ucingo oluyisicaba luklanyelwe ukusiza ukuzinzisa izikhwama ezinomthamo omkhulu, isibonelo izikhwama ezingu-55 oz.

Zonke izikhwama zesampula zesihlungi se-Kedun ziyafaniswa futhi i-DNase-imahhala, i-RNase-imahhala, ayinayo i-Pyrogen.

I-Sponge-Stick esine-10mL Neutralizing Buffer sisehlukanisa ngempela isikhwama sethu sesampula seqoqo, sengeza isendlalelo esengeziwe sokuphepha nokuthembeka enqubweni yakho yokuthatha amasampula. I-Kedun izibophezele ekuletheni izixazululo zekhwalithi ephezulu nezintsha kumakhasimende ethu, futhi isikhwama sethu sokuqoqa isampula simi njengobufakazi balokhu kuzinikela. Enkathini lapho ukunemba nokusebenza kahle kubaluleke kakhulu, isikhwama sokuqoqa isampula sika-Kedun sihlinzeka ngesixazululo esihlanganisa lezi zibaluli emkhiqizweni owodwa, okulula--ukusebenzisa. Ngamagama angaphezu kuka-800 okwethembeka nokwethembeka, sikumema ukuthi uzwe ukusebenza okuphezulu kwesipontshi-Stick esine-10mL Neutralizing Buffer. Guqula indlela oqoqa ngayo amasampula akho nge-Kedun - ngoba yonke imininingwane ibalulekile.