Inshamake y'ibisabwa byo gupima mikorobe
2024 - 01 - 24 15:14:33
Ibisabwa n'intambwe zakwipimisha mikorobeni ibi bikurikira:
Ibisabwa bya Aseptic:
1.Ugomba kwambara imyenda yakazi hamwe numutwe wakazi mugihe utera bagiteri.
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3. Mugihe utanga ibyokurya byintangarugero, oza intoki zawe nisabune mbere yo kwinjira mucyumba cya sterile, hanyuma uhanagure intoki ukoresheje umupira wa pamba wa alcool 75%.
4. Ibyatsi, ibyokurya bisize hamwe nibitangazamakuru byumuco bikoreshwa mugukingira bigomba guhindurwa no guhindurwa. Ibikoresho bidakoreshwa ntibigomba gusigara bidakoreshejwe nyuma yo gufungura paki. Ibikoresho by'ibyuma bigomba kuba autoclave cyangwa gutwikwa inshuro eshatu hamwe na 95% alcool mbere yo kuyikoresha.
5. Iyo ukuye ibyatsi muri paki, isonga yicyatsi ntigomba gukora ku bice byagaragaye. Iyo ukoresheje ibyatsi kugirango utere umuyoboro wikizamini cyangwa isahani, isonga yicyatsi ntigomba gukora ku nkombe yigitereko cyangwa isahani.
6. Gutera ingero no kwimura bagiteri bigomba gukorwa imbere y itara rya alcool. Iyo utera bagiteri cyangwa ingero, ibyatsi bigomba guhindurwa n'umuriro nyuma yo kubikura muri paki hanyuma ugafungura ibizamini byo gupima.
7. Mbere yo gutera za bagiteri, insinga zose zicyuma zo gutera inshinge ninshinge zigomba gutwikwa numuriro, nibiba ngombwa, isano iri hagati yumuzingi, urushinge ninkoni igomba gutwikwa. Inzira yo gutera inshinge za bagiteri yigituntu na bagiteri zifite virusi zigomba gutekwa mumazi abira muminota 5, hanyuma zigatwikwa numuriro.
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Icyumba cyo gukoresha icyumba cya Sterile:
1. Idirishya rigana hanze yicyumba cya sterile rigomba kuba kabiri - risize kandi rifunze. Ntibagomba gukingurwa uko bishakiye. Hagomba kubaho icyumba cya buffer n'inzugi zinyerera zijyanye n'ubunini bw'icyumba cya sterile. Hagomba kandi kuba idirishya rito rya 0.5 ~ 0.7m2. Mugutegura gutambutsa ibintu nyuma yo kwinjira mucyumba cya sterile.
2. Icyumba cya sterile kigomba guhorana isuku. Nyuma yakazi, igomba kwanduzwa hamwe na 2% kugeza 3% yumuti wamasabune ya cresol, ubuso bwakazi bugomba guhanagurwa, nibintu bitajyanye nubushakashatsi ntibigomba kubikwa.
3. Urugi rwicyumba cya sterile rugomba gufungwa cyane mbere na nyuma yo gukoreshwa, kandi itara rya ultraviolet rigomba gucanwa. Niba itara rya ultraviolet ryahagaritswe mu nzu rikoreshwa mu kwanduza, birakenewe itara rya 30W ultraviolet. Intera ni 1.0m, kandi igihe cyo kurasa ntikiri munsi yiminota 30. Iyo ukoresheje amatara ya ultraviolet, ugomba kwitondera Gukora munsi yumucyo ultraviolet kugirango wirinde kwangirika. Itara rigomba guhanagurwa buhoro hamwe nu mupira w’inzoga buri byumweru bibiri kugirango ukureho umukungugu n’amavuta kugirango bigabanye ingaruka ziterwa na ultraviolet.
4. Mugihe utunganya no gutera intanga ibiryo, andika icyumba cya sterile kugirango ukore kandi ntiwemerewe kwinjira cyangwa gusohoka uko bishakiye. Niba ibintu bigomba kunyuzwa hejuru, birashobora kunyuzwa mumadirishya nto.
5. Niba icyuma gikonjesha gikeneye gushyirwaho mucyumba cya sterile, hagomba kubaho igikoresho cyo kuyungurura.
Ibisabwa byo kwanduza no kuboneza urubyaro
Ibirahure, ibikoresho byuma nibitangazamakuru byumuco bikoreshwakwipimisha mikorobe, imico yanduye kandi yatewe, nibindi bigomba guhindurwa mbere yo kubikoresha. Ubushyuhe bwumye nubushyuhe bwumuvuduko wamazi uburyo bwo kuboneza urubyaro.
1. Kwitegura mbere yo kuboneza urubyaro
(1) Ibintu byose bigomba guhindurwa bigomba kubanza gukaraba no gukama. Ibikoresho by'ibirahure nk'ibyatsi n'amasahani meza bigomba kuba bipakiye impapuro. Niba imiyoboro y'icyuma ikoreshwa, imyenge ihumeka kuri yo igomba gukingurwa.
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2. Shyira
(1) Amashanyarazi yumye: Ibintu ntibigomba kuba byuzuye kandi ntibishobora gukora ku nkuta enye z'agasanduku.
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3. Kugenzura ibikoresho
(1) Reba niba icyerekezo cyumuryango cyoroshye, niba impeta ya rubber yangiritse kandi niba iringaniye.
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4. Kuvura Sterilisation
(1) Uburyo bwumye bwo guhagarika ubushyuhe:
Ubu buryo bukwiranye nibintu bitangiza, byangirika, cyangwa bigahumuka mugihe cyubushyuhe bwumye. Irakoreshwa cyane muguhindura ibikoresho byibirahure, ibyuma, ibicuruzwa byubutaka, nibindi.
①Ibikoresho n'ibikoresho bigomba gukaraba mbere yo gukama - guteka kugirango wirinde umwanda ufatiye hejuru kuba karubone.
② Mugihe cyo kuboneza urubyaro, ibintu ntibigomba kuba byuzuye, ntugahure neza nu rukuta rw'agasanduku, hanyuma usige icyuho hagati y'ibintu.
③ Mugihe cyo guhagarika, funga umuryango cyane, uhuze amashanyarazi, fungura umwobo uva muminota igera kuri 30 kugirango ukureho umwuka ukonje muri sterilisateur, uhindure urumuri rwerekana igihe ubushyuhe buzamutse bugera kuri 160 ° C, hanyuma ukomeze kumasaha 1.5 kugeza 2.
FterNyuma yo guhagarika ingero zirangiye cyangwa mugihe cyo kuzamuka kwubushyuhe, urugi rugomba gukingurwa munsi ya 60 ° C.
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① Ongeramo 3L y'amazi mumubiri wingenzi wigikoni cyikurura, na 16L yamazi kumateke uhagaze (umubare wamazi ugomba kuzuzwa mugihe wongeye gukoreshwa, kandi amazi agomba gusimburwa niba ahindutse umuvurungano);
② Kubishobora gutekwa byoroshye, shyiramo umuyoboro usohora hejuru yumupfundikizo wa kaburimbo hejuru kurukuta rwimbere rwikariso ya sterisizasiya (sterilizeri idafite ingofero cyangwa abafite ingofero yangiritse kandi yamenetse ntigomba gukoreshwa);
Gupfuka igifuniko cyo hejuru hanyuma ukizirike cyane kugirango wirinde umwuka; shyira sterilizer kumasoko yumuriro kugirango ushushe, fungura imbaraga zumuriro uhagaze, hanyuma ufungure valve isohoka hejuru yumupfundikizo wo hejuru kugirango urekure umwuka (umwuka uva muminota 10 kugeza kuri 15 amazi amaze kubira));
④Gufunga umuyaga usohora kugirango uzamure umuvuduko wibyuka bisabwa kandi ubigumane mugihe cyagenwe (ukurikije imiterere yibintu byanduye nibihe bijyanye);
⑤ Nyuma yigihe cyagenwe kigeze, kubintu bigomba gukama, hita ufungura valve isohoka kugirango isohore umwuka. Iyo umuvuduko ugarutse kuri zeru, shyira muburyo busanzwe kuri 60 ° C hanyuma ufungure umupfundikizo kugirango ukuremo ibintu. Niba ari ikintu cyamazi, ntukingure umuyaga wuzuye. Inkono igomba kuvanwa mumasoko yubushyuhe ako kanya hanyuma ikemererwa gukonja bisanzwe kugeza igihe umuvuduko ugarutse kuri zeru kandi ubushyuhe bukamanuka munsi ya 60 ° C mbere yo gufungura umupfundikizo kugirango ukuremo ibirimo kugirango wirinde gutungurwa gutunguranye kwamazi gutekwa bikabije cyangwa guturika kwa kontineri.
(3) Kurikiza intambwe zikurikira kugirango ukoreshe igitutu cya horizontal gutekesha amavuta sterilizer:
Funga umuryango w'inkono neza, fungura indege yinjira mu kirere, winjize umwuka muri mezzanine kugirango ushushe, kandi umwuka ukonje muri mezzanine uzahita usohoka unyuze kuri bariyeri;
②Nyuma yuko interlayer igeze ku bushyuhe bwateganijwe, fungura umwuka winjira mu cyumba cy’inkono, winjize umwuka mu cyumba cy’inkono, maze umwuka ukonje mu cyumba cy’inkono uzahita usohoka binyuze mu cyumba cy’icyumba cyafashwe;
HenIyo umuvuduko n'ubushyuhe mucyumba cy'inkono bigeze ku gipimo cyagenwe, hindura indege yinjira mu kirere kugirango ikomeze;
Hisha ubushyuhe bisanzwe cyangwa ubuhanga kuri 60 ℃ mbere yo gufungura umuryango kugirango ukuremo ibintu. Ntugakoreshe uburyo bwihuse bwo gusohora ibyuka kugirango wirinde kugabanuka gutunguranye, guteka bikabije byamazi cyangwa guturika kwa kontineri;
⑤ Iyo ukoresheje progaramu yikora - igenzurwa nigitutu cyamazi sterilizer, nyuma yo gushyira ibintu no gufunga urugi neza, icyerekezo gikwiye kigomba gukanda ukurikije icyiciro cyibintu kugirango sterisizasiya ihite ikorwa muburyo bukenewe. Mugihe cyo kuboneza urubyaro, igikoresho gifatanye kigomba gukoreshwa kugirango bandike ubushyuhe nigihe. Kubireba ejo hazaza, ibisabwa mubikorwa bigomba kuba bikurikije amabwiriza yabakozwe;
5. Ubushyuhe bwa Sterilisation hamwe nigihe
(1) Ubushyuhe bwa Sterilisation yubushyuhe bwumye ni 160 ℃, 1.5 ~ 2h.
(2) Ubushyuhe bwa Sterilisation hamwe nigihe cyumuvuduko wamazi
Uburyo bumwe bwo kuboneza urubyaro
1. Uburyo bwo kuboneza urubyaro:
Koresha sterisizione idafite ingufu. Ibintu bimwe byangizwa byoroshye na - igitutu cyumuvuduko ukabije, ubwo buryo rero burashobora gukoreshwa muguhagarika.
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2. Uburyo bwo gukoresha serumu coagulator:
Iyo umuco wumuco urimo ibintu byihariye nka serumu cyangwa amagi, ubushyuhe bwinshi buzangiza intungamubiri zabwo. Kubwibyo, ubushyuhe buke burashobora gukoreshwa muguhuza serumu no kugera kubikorwa byo kuboneza urubyaro:
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(2) Kora umuco uciriritse ahantu hahanamye cyangwa murwego rwo hejuru nkuko bisabwa. Nyuma yo kongeramo amazi ahagije, huza amashanyarazi, uzamure ubushyuhe kuri 75 ~ 90 ° C kumasaha imwe hanyuma uyihagarike. Shyira muri incubator ya 37 ° C ijoro ryose, hanyuma uyihagarike inshuro eshatu.
3. Guteka no kwanduza:
Urashobora gukoresha inkono itetse cyangwa sterilizer itetse. Amazi amaze kubira, ubiteke muminota 5 kugeza kuri 15. Urashobora kandi kongeramo 2% acide karbolic mumazi hanyuma ukayitekesha muminota 5. Ongeramo 0,02% formaldehyde hanyuma ubiteke kuri 80 ° C muminota 60 kugirango ugere kumugambi wo kuboneza urubyaro. Ariko, guteka sterilisation irashobora kugerwaho. Mugihe ukoresheje ibizamura, hagomba kwitonderwa kubora ibintu.
4. Kuvura Sterilisation:
Nyuma yo kuboneza urubyaro, ibintu bimaze kuba sterile mubihe bisanzwe. Bagomba kugenzurwa neza no gushyirwa mugihe bakuwe muri sterilizer kugirango birinde re - kwanduza;
(1) Kuramo ibintu hanyuma ugenzure ubunyangamugayo bwo gupakira ako kanya. Niba hari ibyangiritse cyangwa tampon ikuweho, ntishobora gukoreshwa nkibintu bidafite ishingiro;
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(3) Umuco urwego cyangwa reagent bigomba kugenzurwa kugirango harebwe niba bihuye ibara cyangwa leta nyuma yo kuboneza urubyaro. Abatujuje ibyangombwa bagomba gutabwa;
(4) Gufungura - gufunga ibintu, imyobo ya ecran igomba gufungwa mugihe uyisohokanye;
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(7) Ibintu byose byujuje ibyangombwa bigomba gushyirwaho itariki yo guhagarika ingengabihe n'itariki izarangiriraho;
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Ibisabwa mu gutunganya imyanda yubumara na bagiteri
Ibikoresho byubushakashatsi numuco bikoreshwa mubushakashatsi bwa mikorobi ntibigomba gukurwa muri laboratoire nta kwanduza.
1. Ibikoresho byanduye byanduye hamwe n imyanda bigomba gushyirwa mubintu bikomeye cyangwa ibitebo byinsinga hanyuma bikabikwa hagati yabigenewe kugeza byashyizweho autoclave imwe.
2. Imico yandujwe na mikorobe igomba kuba autoclave kuri 121 ° C muminota 30.
3. Nyuma yo kuyikoresha, ibyatsi byanduye bigomba gushyirwa mumuti wa 5% ya sool ya creol cyangwa acide acide karbolic, gushiramo byibuze amasaha 24 (amazi yica udukoko ntagomba kuba munsi yuburebure bwokunywa), hanyuma akayungurura umuvuduko mwinshi kuri 121 ° C muminota 30.
4. Amazi yo gusiga no kwoza amavuta arashobora guhita ajugunywa mumazi. Amazi yogeje ya bagiteri zifite virusi agomba guhanagurwa muri beaker hanyuma akayungurura umuvuduko mwinshi mbere yo gusukwa mu miyoboro. Igice cyanditseho gishyirwa mumisabune ya cresol 5%. Nyuma yo gushiramo amazi mumasaha 24, guteka no gukaraba. Amashusho cyangwa amasahani akoreshwa mugupima agglutination agomba kuba autoclave hanyuma akakaraba.
5. Nyuma yo guca umuco, hita utera hanyuma ushire ibice byanduye hamwe na 5% yumuti wamasabune ya cresol cyangwa acide acide kariboli, shiramo igice cyisaha hanyuma uhanagure neza.
Imyenda y'akazi yanduye cyangwa imyenda y'akazi, ingofero, masike, n'ibindi byambarwa mu bizamini by'urugomo bigomba gushyirwa mu mifuka idasanzwe yo kwanduza no kwanduzwa n'umuvuduko mwinshi mbere yo gukaraba.
Ibisabwa byo kwitegura hagati
Ubwiza bwumuco gutegurwa biciriritse bizagira ingaruka ku mikurire y’imikorobe, kubera ko ibinyabuzima bitandukanye bifite imirire itandukanye kandi bigamije umuco. Ibisabwa kugirango ibitangazamakuru bitandukanye byumuco nibi bikurikira:
1. Gupima ibiyigize ukurikije formulaire yo hagati, hanyuma ubishongeshe mumazi yatoboye. Ubwiza bwa reagent ikoreshwa nibiyobyabwenge bigomba kugenzurwa mbere yo kubikoresha.
2. Gupima pH no kubihindura: gupima pH bigomba gukorwa mugihe umuco wumuco ukonje kubushyuhe bwicyumba, kuko hariho itandukaniro runaka muri pH mubihe bishyushye cyangwa ubukonje. Ibipimo birangiye, ongeramo alkali cyangwa aside ukurikije umubare wabazwe hanyuma uvange neza. Bikwiye kongera kugeragezwa. Agaciro pH k'umuco urwego rugomba kuba rwukuri, bitabaye ibyo bizagira ingaruka kumikurire ya mikorobe cyangwa bigira ingaruka kubireba ibisubizo. Ariko, twakagombye kumenya ko - guhagarika igitutu kinini bishobora kugira ingaruka ku kugabanuka cyangwa kwiyongera kwa pH yibitangazamakuru bimwe na bimwe by’umuco, ntabwo rero ari byiza guhagarika ingero nyinshi cyane cyangwa inshuro nyinshi kugirango wirinde kugira ingaruka ku bwiza bw’umuco. Ibipimo, sodium deoxycholate, agar, nibindi muri rusange Ongeraho nyuma yo guhindura pH.
3. Umuco urwego rugomba gusobanurwa neza kugirango byoroherezwe kureba imikurire ya bagiteri. Umuco umaze gushyuha no gutekwa, urashobora kuyungurura ipamba cyangwa flannel ikuramo kugirango ikureho imyanda. Bibaye ngombwa, birashobora gusobanurwa numweru w'igi. Imirongo ya agar yakoreshejwe igomba gukaraba no gukama mbere. Mbere yo gukoresha, irinde kugira ingaruka kumucyo kubera umwanda muri agar.
4. Ibyuma, umuringa nibindi bikoresho ntibigomba gukoreshwa kugirango umuco ugerweho. Nibyiza gukoresha ibikoresho bisukuye bidafite aho bibogamiye.
5. Guhindura umuco w’umuco ntibigomba kugera gusa ku ntego yo kuboneza urubyaro gusa, ahubwo binitondere kutagabanya agaciro k’imirire kubera gushyuha. Mubisanzwe, 121 ° C muminota 15 birahagije. Kubitangazamakuru byumuco birimo ibintu bitihanganira ubushyuhe bwinshi nka sukari na serumu, gelatine, nibindi, bigomba guhindurwa nubushyuhe buke cyangwa uburyo bwigihe gito. Reagent zimwe zidashobora gushyuha, nka potasiyumu tellurite, umuhondo w amagi, TTC, antibiotique, nibindi, bigomba gukonjeshwa kugeza kuri 50 ° C nyuma yuko agar shingiro yashyizweho hanyuma ikongerwamo;
6. Nyuma yicyiciro cyose cyitangazamakuru ryumuco rimaze gutegurwa, hagomba gukorwa ikizamini cyo gukura kidasanzwe hamwe nikizamini cyo gukura cyimiterere yapimwe. Niba ari umuco wa biohimiki, koresha imiti isanzwe ya bagiteri kugirango itere n'umuco, kandi urebe ibisubizo bya biohimiki. Igomba kwerekana reaction isanzwe. Umuco wumuco ntugomba kubikwa igihe kirekire. Bibaye ngombwa, irashobora kubikwa muri firigo ya 4 ° C.
7. Kugeza ubu, hari ubwoko bwinshi bwitangazamakuru ryumuco wumye. Buri cyiciro gikeneye gukoresha imbaraga zisanzwe mugupima gukura cyangwa kwitegereza ibinyabuzima. Ibitangazamakuru bitandukanye byumuco birashobora gukoreshwa nyuma yikizamini cyo gukura kwimiterere ihuye nibyiza. Byaguzwe bishya cyangwa byumye bibitswe igihe kirekire birashobora gukoreshwa. PH yumuco wo hagati nayo igomba gupimwa mugihe uyitegura, kandi dosiye nuburyo bigomba gukurikizwa ukurikije amabwiriza yibicuruzwa.
8. Imiti y’imiti, uburyo bwo kuboneza urubyaro, ibisubizo by’ikizamini cyo gukura, hamwe n’abakozi bashinzwe umusaruro ukoreshwa muri buri cyiciro cy’itangazamakuru ry’umuco ryateguwe bagomba kwandikwa kugira ngo bakore iperereza.
Icyitegererezo cyo gukusanya no gutunganya ibisabwa
1. Ingero zubugenzuzi zegeranijwe zigomba kuba zihagarariye. Mugihe cyo gutoranya, ibikoresho fatizo, gutunganya, gutwara, uburyo bwo kubika nuburyo ibintu byifunguro ryibiryo, ubuzima bw’ibidukikije bikikije ibidukikije, nibindi bigomba gukorwaho ubushakashatsi burambuye kugirango harebwe niba hari aho bituruka.
2. Ukurikije ubwoko nubwinshi bwibiryo, ingano nuburyo byakorwaga bigomba gukorwa hubahirijwe ibisabwa muburyo busanzwe bwo kugenzura.
3. Witondere imikorere ya aseptic mugihe icyitegererezo, kandi kontineri igomba guhindurwa kugirango wirinde kwanduza mikorobe mubidukikije. Ikonteneri ntigomba guhindurwa hamwe nigisubizo cyisabune ya creosal, sterilisation hamwe na chlormethionine, inzoga nizindi zanduza, kandi ntigomba kuba irimo imiti yica udukoko cyangwa antibiotike. Kugirango wirinde kwica mikorobe mu cyitegererezo, imikasi, ibyuma, n'ibiyiko byakoreshejwe nabyo bigomba guhindurwa mbere yo kubikoresha.
4. Ingero zigomba koherezwa mucyumba cyubugenzuzi kugirango zigenzurwe nyuma yo gukusanya. Igenzura muri rusange ntirenza amasaha 3. Niba intera ari ndende, irashobora kubikwa mubidukikije bya 1 ~ 5 ℃. Niba ikeneye gukonjeshwa, igomba kubikwa muburyo bukonje. Tanga igenzura.
5. Nyuma yo kwakira icyitegererezo, icyumba cyubugenzuzi kiziyandikisha (izina ryicyitegererezo, igice cyo kugenzura, ingano, itariki, umubare, nibindi) kandi urebe uko bigaragara byintangarugero. Niba hari kimwe muri ibi bikurikira kibonetse, ubugenzuzi bushobora kwangwa:
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(3) Ingano y'icyitegererezo ntabwo ihagije nkuko bisabwa;
Ingero zatanzwe kugirango zigenzurwe zujuje ibisabwa, icyumba cyubugenzuzi kigomba guhita gikora igenzura nyuma yo kuyakira. Niba ibisabwa bitujujwe, bigomba kubikwa muri firigo ya 4 ° C, kwitegura gukora ibihe mugihe, hanyuma bigakora ubugenzuzi.
6. Mugihe ugenzura ingero, kora gutunganya ukurikije imiterere yabo itandukanye.
(1) Mugihe utanga ingero zamazi, zigomba kuvangwa neza no guterwa ukurikije umubare.
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Icyitegererezo cyo kugenzura, gufata amajwi no gutanga raporo
1. Nyuma yo kubona icyitegererezo, icyumba cyubugenzuzi kizabanza gukora igenzura kandi kigahita gikora igenzura hakurikijwe uburyo rusange bwo kugenzura. Mugihe cyo kugenzura, ibikorwa bya aseptic bigomba gukorwa neza, byitondewe, kandi bikomeye kugirango wirindeibisubizo bya mikorobekwanduza ibidukikije.
2. Uburyo bwakoreshejwe mugihe cyo kugenzura icyitegererezo, ibyabaye nibisubizo byabaye, nibindi bigomba kwandikwa mubizamini byanditse nkibishingiro byo gusesengura no guca ibisubizo. Inyandiko zigomba kuba zirambuye, zisobanutse, zukuri, zifite intego, kandi ntizigomba guhinduka cyangwa guhimbwa.
Ese?
Ese?
Ibisabwa bya Aseptic:
1.Ugomba kwambara imyenda yakazi hamwe numutwe wakazi mugihe utera bagiteri.
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3. Mugihe utanga ibyokurya byintangarugero, oza intoki zawe nisabune mbere yo kwinjira mucyumba cya sterile, hanyuma uhanagure intoki ukoresheje umupira wa pamba wa alcool 75%.
4. Ibyatsi, ibyokurya bisize hamwe nibitangazamakuru byumuco bikoreshwa mugukingira bigomba guhindurwa no guhindurwa. Ibikoresho bidakoreshwa ntibigomba gusigara bidakoreshejwe nyuma yo gufungura paki. Ibikoresho by'ibyuma bigomba kuba autoclave cyangwa gutwikwa inshuro eshatu hamwe na 95% alcool mbere yo kuyikoresha.
5. Iyo ukuye ibyatsi muri paki, isonga yicyatsi ntigomba gukora ku bice byagaragaye. Iyo ukoresheje ibyatsi kugirango utere umuyoboro wikizamini cyangwa isahani, isonga yicyatsi ntigomba gukora ku nkombe yigitereko cyangwa isahani.
6. Gutera ingero no kwimura bagiteri bigomba gukorwa imbere y itara rya alcool. Iyo utera bagiteri cyangwa ingero, ibyatsi bigomba guhindurwa n'umuriro nyuma yo kubikura muri paki hanyuma ugafungura ibizamini byo gupima.
7. Mbere yo gutera za bagiteri, insinga zose zicyuma zo gutera inshinge ninshinge zigomba gutwikwa numuriro, nibiba ngombwa, isano iri hagati yumuzingi, urushinge ninkoni igomba gutwikwa. Inzira yo gutera inshinge za bagiteri yigituntu na bagiteri zifite virusi zigomba gutekwa mumazi abira muminota 5, hanyuma zigatwikwa numuriro.
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Icyumba cyo gukoresha icyumba cya Sterile:
1. Idirishya rigana hanze yicyumba cya sterile rigomba kuba kabiri - risize kandi rifunze. Ntibagomba gukingurwa uko bishakiye. Hagomba kubaho icyumba cya buffer n'inzugi zinyerera zijyanye n'ubunini bw'icyumba cya sterile. Hagomba kandi kuba idirishya rito rya 0.5 ~ 0.7m2. Mugutegura gutambutsa ibintu nyuma yo kwinjira mucyumba cya sterile.
2. Icyumba cya sterile kigomba guhorana isuku. Nyuma yakazi, igomba kwanduzwa hamwe na 2% kugeza 3% yumuti wamasabune ya cresol, ubuso bwakazi bugomba guhanagurwa, nibintu bitajyanye nubushakashatsi ntibigomba kubikwa.
3. Urugi rwicyumba cya sterile rugomba gufungwa cyane mbere na nyuma yo gukoreshwa, kandi itara rya ultraviolet rigomba gucanwa. Niba itara rya ultraviolet ryahagaritswe mu nzu rikoreshwa mu kwanduza, birakenewe itara rya 30W ultraviolet. Intera ni 1.0m, kandi igihe cyo kurasa ntikiri munsi yiminota 30. Iyo ukoresheje amatara ya ultraviolet, ugomba kwitondera Gukora munsi yumucyo ultraviolet kugirango wirinde kwangirika. Itara rigomba guhanagurwa buhoro hamwe nu mupira w’inzoga buri byumweru bibiri kugirango ukureho umukungugu n’amavuta kugirango bigabanye ingaruka ziterwa na ultraviolet.
4. Mugihe utunganya no gutera intanga ibiryo, andika icyumba cya sterile kugirango ukore kandi ntiwemerewe kwinjira cyangwa gusohoka uko bishakiye. Niba ibintu bigomba kunyuzwa hejuru, birashobora kunyuzwa mumadirishya nto.
5. Niba icyuma gikonjesha gikeneye gushyirwaho mucyumba cya sterile, hagomba kubaho igikoresho cyo kuyungurura.
Ibisabwa byo kwanduza no kuboneza urubyaro
Ibirahure, ibikoresho byuma nibitangazamakuru byumuco bikoreshwakwipimisha mikorobe, imico yanduye kandi yatewe, nibindi bigomba guhindurwa mbere yo kubikoresha. Ubushyuhe bwumye nubushyuhe bwumuvuduko wamazi uburyo bwo kuboneza urubyaro.
1. Kwitegura mbere yo kuboneza urubyaro
(1) Ibintu byose bigomba guhindurwa bigomba kubanza gukaraba no gukama. Ibikoresho by'ibirahure nk'ibyatsi n'amasahani meza bigomba kuba bipakiye impapuro. Niba imiyoboro y'icyuma ikoreshwa, imyenge ihumeka kuri yo igomba gukingurwa.
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2. Shyira
(1) Amashanyarazi yumye: Ibintu ntibigomba kuba byuzuye kandi ntibishobora gukora ku nkuta enye z'agasanduku.
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3. Kugenzura ibikoresho
(1) Reba niba icyerekezo cyumuryango cyoroshye, niba impeta ya rubber yangiritse kandi niba iringaniye.
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4. Kuvura Sterilisation
(1) Uburyo bwumye bwo guhagarika ubushyuhe:
Ubu buryo bukwiranye nibintu bitangiza, byangirika, cyangwa bigahumuka mugihe cyubushyuhe bwumye. Irakoreshwa cyane muguhindura ibikoresho byibirahure, ibyuma, ibicuruzwa byubutaka, nibindi.
①Ibikoresho n'ibikoresho bigomba gukaraba mbere yo gukama - guteka kugirango wirinde umwanda ufatiye hejuru kuba karubone.
② Mugihe cyo kuboneza urubyaro, ibintu ntibigomba kuba byuzuye, ntugahure neza nu rukuta rw'agasanduku, hanyuma usige icyuho hagati y'ibintu.
③ Mugihe cyo guhagarika, funga umuryango cyane, uhuze amashanyarazi, fungura umwobo uva muminota igera kuri 30 kugirango ukureho umwuka ukonje muri sterilisateur, uhindure urumuri rwerekana igihe ubushyuhe buzamutse bugera kuri 160 ° C, hanyuma ukomeze kumasaha 1.5 kugeza 2.
FterNyuma yo guhagarika ingero zirangiye cyangwa mugihe cyo kuzamuka kwubushyuhe, urugi rugomba gukingurwa munsi ya 60 ° C.
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① Ongeramo 3L y'amazi mumubiri wingenzi wigikoni cyikurura, na 16L yamazi kumateke uhagaze (umubare wamazi ugomba kuzuzwa mugihe wongeye gukoreshwa, kandi amazi agomba gusimburwa niba ahindutse umuvurungano);
② Kubishobora gutekwa byoroshye, shyiramo umuyoboro usohora hejuru yumupfundikizo wa kaburimbo hejuru kurukuta rwimbere rwikariso ya sterisizasiya (sterilizeri idafite ingofero cyangwa abafite ingofero yangiritse kandi yamenetse ntigomba gukoreshwa);
Gupfuka igifuniko cyo hejuru hanyuma ukizirike cyane kugirango wirinde umwuka; shyira sterilizer kumasoko yumuriro kugirango ushushe, fungura imbaraga zumuriro uhagaze, hanyuma ufungure valve isohoka hejuru yumupfundikizo wo hejuru kugirango urekure umwuka (umwuka uva muminota 10 kugeza kuri 15 amazi amaze kubira));
④Gufunga umuyaga usohora kugirango uzamure umuvuduko wibyuka bisabwa kandi ubigumane mugihe cyagenwe (ukurikije imiterere yibintu byanduye nibihe bijyanye);
⑤ Nyuma yigihe cyagenwe kigeze, kubintu bigomba gukama, hita ufungura valve isohoka kugirango isohore umwuka. Iyo umuvuduko ugarutse kuri zeru, shyira muburyo busanzwe kuri 60 ° C hanyuma ufungure umupfundikizo kugirango ukuremo ibintu. Niba ari ikintu cyamazi, ntukingure umuyaga wuzuye. Inkono igomba kuvanwa mumasoko yubushyuhe ako kanya hanyuma ikemererwa gukonja bisanzwe kugeza igihe umuvuduko ugarutse kuri zeru kandi ubushyuhe bukamanuka munsi ya 60 ° C mbere yo gufungura umupfundikizo kugirango ukuremo ibirimo kugirango wirinde gutungurwa gutunguranye kwamazi gutekwa bikabije cyangwa guturika kwa kontineri.
(3) Kurikiza intambwe zikurikira kugirango ukoreshe igitutu cya horizontal gutekesha amavuta sterilizer:
Funga umuryango w'inkono neza, fungura indege yinjira mu kirere, winjize umwuka muri mezzanine kugirango ushushe, kandi umwuka ukonje muri mezzanine uzahita usohoka unyuze kuri bariyeri;
②Nyuma yuko interlayer igeze ku bushyuhe bwateganijwe, fungura umwuka winjira mu cyumba cy’inkono, winjize umwuka mu cyumba cy’inkono, maze umwuka ukonje mu cyumba cy’inkono uzahita usohoka binyuze mu cyumba cy’icyumba cyafashwe;
HenIyo umuvuduko n'ubushyuhe mucyumba cy'inkono bigeze ku gipimo cyagenwe, hindura indege yinjira mu kirere kugirango ikomeze;
Hisha ubushyuhe bisanzwe cyangwa ubuhanga kuri 60 ℃ mbere yo gufungura umuryango kugirango ukuremo ibintu. Ntugakoreshe uburyo bwihuse bwo gusohora ibyuka kugirango wirinde kugabanuka gutunguranye, guteka bikabije byamazi cyangwa guturika kwa kontineri;
⑤ Iyo ukoresheje progaramu yikora - igenzurwa nigitutu cyamazi sterilizer, nyuma yo gushyira ibintu no gufunga urugi neza, icyerekezo gikwiye kigomba gukanda ukurikije icyiciro cyibintu kugirango sterisizasiya ihite ikorwa muburyo bukenewe. Mugihe cyo kuboneza urubyaro, igikoresho gifatanye kigomba gukoreshwa kugirango bandike ubushyuhe nigihe. Kubireba ejo hazaza, ibisabwa mubikorwa bigomba kuba bikurikije amabwiriza yabakozwe;
5. Ubushyuhe bwa Sterilisation hamwe nigihe
(1) Ubushyuhe bwa Sterilisation yubushyuhe bwumye ni 160 ℃, 1.5 ~ 2h.
(2) Ubushyuhe bwa Sterilisation hamwe nigihe cyumuvuduko wamazi
Uburyo bumwe bwo kuboneza urubyaro
1. Uburyo bwo kuboneza urubyaro:
Koresha sterisizione idafite ingufu. Ibintu bimwe byangizwa byoroshye na - igitutu cyumuvuduko ukabije, ubwo buryo rero burashobora gukoreshwa muguhagarika.
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. Kora ibi inshuro eshatu kugirango ugere ku ntego yo kuboneza urubyaro.
2. Uburyo bwo gukoresha serumu coagulator:
Iyo umuco wumuco urimo ibintu byihariye nka serumu cyangwa amagi, ubushyuhe bwinshi buzangiza intungamubiri zabwo. Kubwibyo, ubushyuhe buke burashobora gukoreshwa muguhuza serumu no kugera kubikorwa byo kuboneza urubyaro:
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(2) Kora umuco uciriritse ahantu hahanamye cyangwa murwego rwo hejuru nkuko bisabwa. Nyuma yo kongeramo amazi ahagije, huza amashanyarazi, uzamure ubushyuhe kuri 75 ~ 90 ° C kumasaha imwe hanyuma uyihagarike. Shyira muri incubator ya 37 ° C ijoro ryose, hanyuma uyihagarike inshuro eshatu.
3. Guteka no kwanduza:
Urashobora gukoresha inkono itetse cyangwa sterilizer itetse. Amazi amaze kubira, ubiteke muminota 5 kugeza kuri 15. Urashobora kandi kongeramo 2% acide karbolic mumazi hanyuma ukayitekesha muminota 5. Ongeramo 0,02% formaldehyde hanyuma ubiteke kuri 80 ° C muminota 60 kugirango ugere kumugambi wo kuboneza urubyaro. Ariko, guteka sterilisation irashobora kugerwaho. Mugihe ukoresheje ibizamura, hagomba kwitonderwa kubora ibintu.
4. Kuvura Sterilisation:
Nyuma yo kuboneza urubyaro, ibintu bimaze kuba sterile mubihe bisanzwe. Bagomba kugenzurwa neza no gushyirwa mugihe bakuwe muri sterilizer kugirango birinde re - kwanduza;
(1) Kuramo ibintu hanyuma ugenzure ubunyangamugayo bwo gupakira ako kanya. Niba hari ibyangiritse cyangwa tampon ikuweho, ntishobora gukoreshwa nkibintu bidafite ishingiro;
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(3) Umuco urwego cyangwa reagent bigomba kugenzurwa kugirango harebwe niba bihuye ibara cyangwa leta nyuma yo kuboneza urubyaro. Abatujuje ibyangombwa bagomba gutabwa;
(4) Gufungura - gufunga ibintu, imyobo ya ecran igomba gufungwa mugihe uyisohokanye;
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(7) Ibintu byose byujuje ibyangombwa bigomba gushyirwaho itariki yo guhagarika ingengabihe n'itariki izarangiriraho;
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Ibisabwa mu gutunganya imyanda yubumara na bagiteri
Ibikoresho byubushakashatsi numuco bikoreshwa mubushakashatsi bwa mikorobi ntibigomba gukurwa muri laboratoire nta kwanduza.
1. Ibikoresho byanduye byanduye hamwe n imyanda bigomba gushyirwa mubintu bikomeye cyangwa ibitebo byinsinga hanyuma bikabikwa hagati yabigenewe kugeza byashyizweho autoclave imwe.
2. Imico yandujwe na mikorobe igomba kuba autoclave kuri 121 ° C muminota 30.
3. Nyuma yo kuyikoresha, ibyatsi byanduye bigomba gushyirwa mumuti wa 5% ya sool ya creol cyangwa acide acide karbolic, gushiramo byibuze amasaha 24 (amazi yica udukoko ntagomba kuba munsi yuburebure bwokunywa), hanyuma akayungurura umuvuduko mwinshi kuri 121 ° C muminota 30.
4. Amazi yo gusiga no kwoza amavuta arashobora guhita ajugunywa mumazi. Amazi yogeje ya bagiteri zifite virusi agomba guhanagurwa muri beaker hanyuma akayungurura umuvuduko mwinshi mbere yo gusukwa mu miyoboro. Igice cyanditseho gishyirwa mumisabune ya cresol 5%. Nyuma yo gushiramo amazi mumasaha 24, guteka no gukaraba. Amashusho cyangwa amasahani akoreshwa mugupima agglutination agomba kuba autoclave hanyuma akakaraba.
5. Nyuma yo guca umuco, hita utera hanyuma ushire ibice byanduye hamwe na 5% yumuti wamasabune ya cresol cyangwa acide acide kariboli, shiramo igice cyisaha hanyuma uhanagure neza.
Imyenda y'akazi yanduye cyangwa imyenda y'akazi, ingofero, masike, n'ibindi byambarwa mu bizamini by'urugomo bigomba gushyirwa mu mifuka idasanzwe yo kwanduza no kwanduzwa n'umuvuduko mwinshi mbere yo gukaraba.
Ibisabwa byo kwitegura hagati
Ubwiza bwumuco gutegurwa biciriritse bizagira ingaruka ku mikurire y’imikorobe, kubera ko ibinyabuzima bitandukanye bifite imirire itandukanye kandi bigamije umuco. Ibisabwa kugirango ibitangazamakuru bitandukanye byumuco nibi bikurikira:
1. Gupima ibiyigize ukurikije formulaire yo hagati, hanyuma ubishongeshe mumazi yatoboye. Ubwiza bwa reagent ikoreshwa nibiyobyabwenge bigomba kugenzurwa mbere yo kubikoresha.
2. Gupima pH no kubihindura: gupima pH bigomba gukorwa mugihe umuco wumuco ukonje kubushyuhe bwicyumba, kuko hariho itandukaniro runaka muri pH mubihe bishyushye cyangwa ubukonje. Ibipimo birangiye, ongeramo alkali cyangwa aside ukurikije umubare wabazwe hanyuma uvange neza. Bikwiye kongera kugeragezwa. Agaciro pH k'umuco urwego rugomba kuba rwukuri, bitabaye ibyo bizagira ingaruka kumikurire ya mikorobe cyangwa bigira ingaruka kubireba ibisubizo. Ariko, twakagombye kumenya ko - guhagarika igitutu kinini bishobora kugira ingaruka ku kugabanuka cyangwa kwiyongera kwa pH yibitangazamakuru bimwe na bimwe by’umuco, ntabwo rero ari byiza guhagarika ingero nyinshi cyane cyangwa inshuro nyinshi kugirango wirinde kugira ingaruka ku bwiza bw’umuco. Ibipimo, sodium deoxycholate, agar, nibindi muri rusange Ongeraho nyuma yo guhindura pH.
3. Umuco urwego rugomba gusobanurwa neza kugirango byoroherezwe kureba imikurire ya bagiteri. Umuco umaze gushyuha no gutekwa, urashobora kuyungurura ipamba cyangwa flannel ikuramo kugirango ikureho imyanda. Bibaye ngombwa, birashobora gusobanurwa numweru w'igi. Imirongo ya agar yakoreshejwe igomba gukaraba no gukama mbere. Mbere yo gukoresha, irinde kugira ingaruka kumucyo kubera umwanda muri agar.
4. Ibyuma, umuringa nibindi bikoresho ntibigomba gukoreshwa kugirango umuco ugerweho. Nibyiza gukoresha ibikoresho bisukuye bidafite aho bibogamiye.
5. Guhindura umuco w’umuco ntibigomba kugera gusa ku ntego yo kuboneza urubyaro gusa, ahubwo binitondere kutagabanya agaciro k’imirire kubera gushyuha. Mubisanzwe, 121 ° C muminota 15 birahagije. Kubitangazamakuru byumuco birimo ibintu bitihanganira ubushyuhe bwinshi nka sukari na serumu, gelatine, nibindi, bigomba guhindurwa nubushyuhe buke cyangwa uburyo bwigihe gito. Reagent zimwe zidashobora gushyuha, nka potasiyumu tellurite, umuhondo w amagi, TTC, antibiotique, nibindi, bigomba gukonjeshwa kugeza kuri 50 ° C nyuma yuko agar shingiro yashyizweho hanyuma ikongerwamo;
6. Nyuma yicyiciro cyose cyitangazamakuru ryumuco rimaze gutegurwa, hagomba gukorwa ikizamini cyo gukura kidasanzwe hamwe nikizamini cyo gukura cyimiterere yapimwe. Niba ari umuco wa biohimiki, koresha imiti isanzwe ya bagiteri kugirango itere n'umuco, kandi urebe ibisubizo bya biohimiki. Igomba kwerekana reaction isanzwe. Umuco wumuco ntugomba kubikwa igihe kirekire. Bibaye ngombwa, irashobora kubikwa muri firigo ya 4 ° C.
7. Kugeza ubu, hari ubwoko bwinshi bwitangazamakuru ryumuco wumye. Buri cyiciro gikeneye gukoresha imbaraga zisanzwe mugupima gukura cyangwa kwitegereza ibinyabuzima. Ibitangazamakuru bitandukanye byumuco birashobora gukoreshwa nyuma yikizamini cyo gukura kwimiterere ihuye nibyiza. Byaguzwe bishya cyangwa byumye bibitswe igihe kirekire birashobora gukoreshwa. PH yumuco wo hagati nayo igomba gupimwa mugihe uyitegura, kandi dosiye nuburyo bigomba gukurikizwa ukurikije amabwiriza yibicuruzwa.
8. Imiti y’imiti, uburyo bwo kuboneza urubyaro, ibisubizo by’ikizamini cyo gukura, hamwe n’abakozi bashinzwe umusaruro ukoreshwa muri buri cyiciro cy’itangazamakuru ry’umuco ryateguwe bagomba kwandikwa kugira ngo bakore iperereza.
Icyitegererezo cyo gukusanya no gutunganya ibisabwa
1. Ingero zubugenzuzi zegeranijwe zigomba kuba zihagarariye. Mugihe cyo gutoranya, ibikoresho fatizo, gutunganya, gutwara, uburyo bwo kubika nuburyo ibintu byifunguro ryibiryo, ubuzima bw’ibidukikije bikikije ibidukikije, nibindi bigomba gukorwaho ubushakashatsi burambuye kugirango harebwe niba hari aho bituruka.
2. Ukurikije ubwoko nubwinshi bwibiryo, ingano nuburyo byakorwaga bigomba gukorwa hubahirijwe ibisabwa muburyo busanzwe bwo kugenzura.
3. Witondere imikorere ya aseptic mugihe icyitegererezo, kandi kontineri igomba guhindurwa kugirango wirinde kwanduza mikorobe mubidukikije. Ikonteneri ntigomba guhindurwa hamwe nigisubizo cyisabune ya creosal, sterilisation hamwe na chlormethionine, inzoga nizindi zanduza, kandi ntigomba kuba irimo imiti yica udukoko cyangwa antibiotike. Kugirango wirinde kwica mikorobe mu cyitegererezo, imikasi, ibyuma, n'ibiyiko byakoreshejwe nabyo bigomba guhindurwa mbere yo kubikoresha.
4. Ingero zigomba koherezwa mucyumba cyubugenzuzi kugirango zigenzurwe nyuma yo gukusanya. Igenzura muri rusange ntirenza amasaha 3. Niba intera ari ndende, irashobora kubikwa mubidukikije bya 1 ~ 5 ℃. Niba ikeneye gukonjeshwa, igomba kubikwa muburyo bukonje. Tanga igenzura.
5. Nyuma yo kwakira icyitegererezo, icyumba cyubugenzuzi kiziyandikisha (izina ryicyitegererezo, igice cyo kugenzura, ingano, itariki, umubare, nibindi) kandi urebe uko bigaragara byintangarugero. Niba hari kimwe muri ibi bikurikira kibonetse, ubugenzuzi bushobora kwangwa:
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(3) Ingano y'icyitegererezo ntabwo ihagije nkuko bisabwa;
Ingero zatanzwe kugirango zigenzurwe zujuje ibisabwa, icyumba cyubugenzuzi kigomba guhita gikora igenzura nyuma yo kuyakira. Niba ibisabwa bitujujwe, bigomba kubikwa muri firigo ya 4 ° C, kwitegura gukora ibihe mugihe, hanyuma bigakora ubugenzuzi.
6. Mugihe ugenzura ingero, kora gutunganya ukurikije imiterere yabo itandukanye.
(1) Mugihe utanga ingero zamazi, zigomba kuvangwa neza no guterwa ukurikije umubare.
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Icyitegererezo cyo kugenzura, gufata amajwi no gutanga raporo
1. Nyuma yo kubona icyitegererezo, icyumba cyubugenzuzi kizabanza gukora igenzura kandi kigahita gikora igenzura hakurikijwe uburyo rusange bwo kugenzura. Mugihe cyo kugenzura, ibikorwa bya aseptic bigomba gukorwa neza, byitondewe, kandi bikomeye kugirango wirindeibisubizo bya mikorobekwanduza ibidukikije.
2. Uburyo bwakoreshejwe mugihe cyo kugenzura icyitegererezo, ibyabaye nibisubizo byabaye, nibindi bigomba kwandikwa mubizamini byanditse nkibishingiro byo gusesengura no guca ibisubizo. Inyandiko zigomba kuba zirambuye, zisobanutse, zukuri, zifite intego, kandi ntizigomba guhinduka cyangwa guhimbwa.
Ese?
Ese?
